Comparative metatranscriptomics reveals widespread community responses during phenanthrene degradation in soil
Identifieur interne : 000605 ( Main/Exploration ); précédent : 000604; suivant : 000606Comparative metatranscriptomics reveals widespread community responses during phenanthrene degradation in soil
Auteurs : Alexandre De Menezes [Irlande (pays)] ; Nicholas Clipson [Irlande (pays)] ; Evelyn Doyle [Irlande (pays)]Source :
- Environmental Microbiology [ 1462-2912 ] ; 2012-09.
Abstract
Soil microbial community response to phenanthrene was evaluated by metatranscriptomics. A marked increase in transcripts involved in aromatic compound metabolism, respiration and stress responses, and concurrent decreases in virulence, carbohydrate, DNA metabolism and phosphorus metabolism transcripts was revealed. Phenanthrene addition led to a 1.8‐fold to 33‐fold increase in the abundance of dioxygenase, stress response and detoxification transcripts, whereas those of general metabolism were little affected. Heavy metal P‐type ATPases and thioredoxin transcripts were more abundant in the phenanthrene‐amended soil, and this is the first time these proteins have been associated with polycyclic aromatic hydrocarbon (PAH) stress in microorganisms. Annotation with custom databases constructed with bacterial or fungal PAH metabolism protein sequences showed that increases in PAH‐degradatory gene expression occurred for all gene groups investigated. Taxonomic determination of mRNA transcripts showed widespread changes in the bacteria, archaea and fungi, and the actinobacteria were responsible for most of the de novo expression of transcripts associated with dioxygenases, stress response and detoxification genes. This is the first report of an experimental metatranscriptomic study detailing microbial community responses to a pollutant in soil, and offers information on novel in situ effects of PAHs on soil microbes that can be explored further.
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DOI: 10.1111/j.1462-2920.2012.02781.x
Affiliations:
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A marked increase in transcripts involved in aromatic compound metabolism, respiration and stress responses, and concurrent decreases in virulence, carbohydrate, DNA metabolism and phosphorus metabolism transcripts was revealed. Phenanthrene addition led to a 1.8‐fold to 33‐fold increase in the abundance of dioxygenase, stress response and detoxification transcripts, whereas those of general metabolism were little affected. Heavy metal P‐type ATPases and thioredoxin transcripts were more abundant in the phenanthrene‐amended soil, and this is the first time these proteins have been associated with polycyclic aromatic hydrocarbon (PAH) stress in microorganisms. Annotation with custom databases constructed with bacterial or fungal PAH metabolism protein sequences showed that increases in PAH‐degradatory gene expression occurred for all gene groups investigated. Taxonomic determination of mRNA transcripts showed widespread changes in the bacteria, archaea and fungi, and the actinobacteria were responsible for most of the de novo expression of transcripts associated with dioxygenases, stress response and detoxification genes. This is the first report of an experimental metatranscriptomic study detailing microbial community responses to a pollutant in soil, and offers information on novel in situ effects of PAHs on soil microbes that can be explored further.</div></front></TEI><affiliations><list><country><li>Irlande (pays)</li></country></list><tree><country name="Irlande (pays)"><noRegion><name sortKey="De Menezes, Alexandre" sort="De Menezes, Alexandre" uniqKey="De Menezes A" first="Alexandre" last="De Menezes">Alexandre De Menezes</name></noRegion><name sortKey="Clipson, Nicholas" sort="Clipson, Nicholas" uniqKey="Clipson N" first="Nicholas" last="Clipson">Nicholas Clipson</name><name sortKey="Doyle, Evelyn" sort="Doyle, Evelyn" uniqKey="Doyle E" first="Evelyn" last="Doyle">Evelyn Doyle</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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